The observation of nerve growth factor and substance Pin synovium of knee osteoarthritis
- VernacularTitle:膝关节骨关节炎滑膜中神经生长因子和P物质水平测试与观察
- Author:
Baicheng CHEN
;
Xiaofeng WANG
;
Jing ZHANG
- Publication Type:Journal Article
- Keywords:
Osteoarthritis, knee;
Synovial membrane;
Nerve growth factors;
Substance
- From:
Chinese Journal of Orthopaedics
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the pathogenesis of pain around the medial site of knee joint in osteoarthritis, the expressions of nerve growth factor (NGF) and substance P (SP) were observed in the synovial membrane of medial, lateral, and suprapatellar sites of knees in osteoarthritis and simple meniscus tear. Methods Synovial tissues were obtained from medial, lateral, and suprapatellar sites of 13 knees (12 patients with an average age of 57 years) with OA at surgery. Synovial tissues as control were harvested from medial, lateral, and suprapatellar sites of 6 knees (6 patients with an average of 52 years) and simple meniscus tear during the arthroscopic operation. The tissues were cut into small fragments, fixed with formalin and then embedded in paraffin. The expressions of NGF and SP in synovium of the knees were detected using immunohistochemical staining. Gless nerve fiber staining was adopted to observe the distribution of nerve fibers in synovium of the knee. Results NGF revealed in granular pattern in the muscular wall of the vessels in synovium. The positive vessel rates of NGF in the medial synovium of knee osteoarthritis (67.2%) were higher than those in lateral synovium (47.6%,P
