Study on the isolation and phenotype of peripheral blood pDC_1 and pDC_2 in rhesus monkey
- VernacularTitle:恒河猴外周血pDC_1/pDC_2的分离和细胞表现型的研究
- Author:
Linyou ZHANG
;
Xuefeng ZHANG
;
Qiuming XIA
- Publication Type:Journal Article
- Keywords:
Macaca;
pDC 1;
pDC2
- From:
Chinese Journal of Organ Transplantation
1996;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the isolation and phenotype of peripheral blood pDC 1 and pDC 2 in rhesus monkey.Methods Peripheral blood monoclear cells (PBMCs) were isolated from healthy, SIV-negative rhesus monkeys (M.mulatta) using Ficoll-Hypaque density separation. DC precursors were identified and were sorted by 3 color rare-event cytometric flow analysis using human monoclonal antibodies cross reactive with rhesus monkey. Results DC subsets were identified within the lineage- HLA-DR+ fraction of PBMCs and maintained activities. Myeloid DC (pDC 1) showed the phenotype lineage-, HLA-DR+ and BDCA 1+; Lymphoid DC (pDC 2)showed the phenotype lineage-, HLA-DR+ and CD123+ (IL-3R?+).Conclusions We have identified the rhesus monkey pDC 1 and pDC 2 similar to those from human beings. Identification of pDC 1 and pDC 2 is an important first step towards testing of these important immunomodulatory APC in the therapy of allograft rejection in non-human primates.
