Glucocorticoid up-regulated T?R-Ⅱ in ovarian cancer HO-8910 cells
- VernacularTitle:糖皮质激素对人卵巢癌HO-8910细胞T?R-Ⅱ的上调作用
- Author:
Bing XIA
;
Jian LU
- Publication Type:Journal Article
- Keywords:
glucocorticoid;
transforming growth factor;
ovarian cancer cell line;
cell proliferation
- From:
China Oncology
1998;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Purpose:To investigate whether transforming growth factor ?1 (TGF ?1) pathway is involved in the mechanism of dexamethasone (Dex) mediated proliferation inhibition in ovarian cancer cell line HO 8910.Methods:To analyse cell proliferation and cell cycle distribution by cell counts and flow cytometric analysis, respectively ; to determine the expression levels of TGF ?1 and its two receptors, T?R Ⅰ and T?R Ⅱ , by quantative RT PCR, ELISA and(or) immunocytochemistry methods.Results:Dex induced a G 0 /G 1 cell cycle arrest in HO 8910 cells, and it up regulated T?R Ⅱ expression in a concentration dependent manner.The level of T?R Ⅱ mRNA was the highest after treatment with Dex for 8 hours, with 1.4 fold more than that of control at concentration of 10 -7 mol/L ( P
