Differentiation of rat bone marrow stromal cells into Schwann cells-like in vitro
- VernacularTitle:大鼠骨髓基质干细胞体外诱导分化为类许旺细胞
- Author:
Saiyun HOU
;
Jiakai ZHU
- Publication Type:Journal Article
- Keywords:
Bone marrow stromal cells;
Induction and differentiation;
Schwann cells;
Rat
- From:
Chinese Journal of Microsurgery
1998;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the protocol that rat bone marrow stromal cells (BMSCs) can be induced to differentiate into Schwann cell-like in vitro. Methods BMSCs were treated with beta-mercaptoethanol followed by retinoic acid and cultured in the presence of forskolin,basic-FGF,PDGF and heregulin. The positive percentages of GFAP,S-100 protein expression were measured by immunocytochemistry SABC staining. Results After the induction,BMSCs displayed morphologies of Schwann cell,such as ellipsoidal cell bodies and fences-like array,with two or three sligh cell processes. The positive percentages of GFAP protein expression was from 42.5% to 68.7%,S-100 was from 39.6% to 60.2%. Conclusions Rat BMSCs could be induced to Schwann cell-like by beta-mercaptoethanol,retinoic acid,forskolin,basic-FGF,PDGF and heregulin
