Surgical technique of the model of orthotopic liver transplantation and prevention of operational complication in rat
- VernacularTitle:大鼠原位肝移植模型的手术技巧及并发症的预防
- Author:
Yi MA
;
Xiaoshun HE
;
Guihua CHEN
- Publication Type:Journal Article
- Keywords:
Liver;
Transplantation;
Microsurgery;
Complication
- From:
Chinese Journal of Microsurgery
1998;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the surgical technique of establishing a reliable rat model of orthotopic liver transplantation and prevention of operational complication. Methods The model was established with modified cuff technique.The donor was perfused through the abdominal aorta with 20ml cold perfusate.The anastomosis of the suprahepatic vena cave(VC)was sutured end-to-end with 8-0 nylon line,and the continuity of infrahepatic VC and portal vein (PV)were established by means of cuff method respectively.The bile duct anastomosis was performed by internal stent. Results Three hundred and sixty case of rat orthotopic liver transplantation were performed and the successful rate of the mode was 91.3%.In non-intervention group,the survival rat was satisfactory,with a 86.5%of 1 week survival and a 80.7% of long-term survival (longer than 3 months). Conclusions The sophisticated microsurgical technique and the delicate surgical manipulation is the prerequisite of preventing operational complication,and shortening the anhepatic phase of recipient as soon as possible is the key to animal survival.
