Screening antigen epitope of bFGF by phage display
- VernacularTitle:噬菌体展示技术筛选bFGF抗原表位
- Author:
Junrjian XIANG
;
Zhenyu ZHONG
;
Hongyu YANG
- Publication Type:Journal Article
- Keywords:
Basic Fibroblast growth factor(bFGF);
Antigen epitope;
Phage display;
Vaccine
- From:
Chinese Journal of Immunology
1985;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the epitope and its immunogenicity of bFGF. Methods -.The phage disply 7 peptides library was screened with monoclonal antibody GE22 to bFGF, which neutralize the bioactivin'es of bFGF. Results: After three cycles screening, the isolated phage clones with GF22 epitopes specifically inhibited bFGF binding to GF22.Sequence analysis showd that the epitopes shared a highly consensus spequence( Leu-Pro-Pro/Leu-Gly-His-Phe/He-Lys) and PPGHFK sequence was located at aino acids 22-27 (PPGHFK) within bFGF (155aa) molecule.Phage clones with the epitopes could highly induce imuno-response in mice,eapically with the sequence LPLGHK was 3 times higher than original sequence. Conclusion:Trie sequence LPLGHK may be a valuable vaccine in future studies of bFGF.
