Construction of a vector suitable for making large phage antibody library
- VernacularTitle:用于大容量噬菌体抗体库的表达载体的构建及鉴定
- Author:
Yon WANG
;
Yuxiao WANG
;
Xiaosid CHEN
- Publication Type:Journal Article
- Keywords:
Phage;
Antibody library;
loxp-cre recombination system;
In vivo recombination
- From:
Chinese Journal of Immunology
1985;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To construct a vector that suits the construction of large phage antibody libraries.Methods:Hie phage antibody vector p3MH was modified by insertion of synthesized oligos and PCR mediated site-specific mutations. Hie resultant vector was checked by expression of phage antibody, ELBA and in vivo recombination. Results: Vector pAL was obtained by following modifications of p3MH: insertion of Ioxp511 and loxp sequences, substitution of Lac promoter by Ara promoter, and modification of the cloning site for antibody genes. pAL was proved capable of expressing functional Fab phage antibodies under tighter control. In ere+ bacteria, pAL exhibited loxp-cre mediated recombination. Conclusion: pAL is useful for construction of large phage antibody libraries.
