Activation of caspase-6 during resveratrol(Res)-induced apoptosis of nasopharyn- geal carcinoma cells CNE-2Z
- VernacularTitle:白藜芦醇诱导鼻咽癌细胞CNE-2Z凋亡过程中caspase-6的活化
- Author:
Xudong TANG
;
Keyuan ZHOU
;
Xiaowen CHEN
- Publication Type:Journal Article
- Keywords:
Resveratrol;
Nasopharyngeal carcinoma cells;
Apoptosis;
Caspase-6
- From:
Chinese Journal of Immunology
1985;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study whether caspase-6 was activated during resveratrol(Res)- induced apoptosis of nasopharyngeal carcinoma cells CNE-2Z. Methods:The cleavage of pro-caspase-6 was analyzed by Western-blot. TTie changes of caspase-6 mKNA was detected by semi-quantitative RT-PCR. The changes of caspase-6 activity was determined by colorimetric assay. Results: After CNE-2Z cells were treated with 0 (control), 25, 50, 100, 200 pmol/L Res for 24 h, respectively, the expression of pro-caspase-6 was decreased with concentration increasing. Caspase-6 active fragment P20(20 kD) appeared at 100 fjtmol/L and was increased at 200 (junol/L.The expression of caspase-6 mR-NA was increased in a concentration-dependent manner ( P
