Orthotopical transplantation of human renal carcinoma tissue into nude mice and the establishment of a high metastatic cell line MRCC
- VernacularTitle:裸鼠人肾癌SOI模型的建立及高转移亚株的筛选
- Author:
Pengfei WANG
;
Changhong SHI
;
Chen SHAO
- Publication Type:Journal Article
- Keywords:
Kidney neoplasms;
Carcinoma;
Cells,cultured
- From:
Chinese Journal of Urology
1994;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a SOI model of human renal carcinoma and a high metastatic cell subline. Methods A human renal cell line RCC-9863 has been established by inoculating a human renal tumor tissue into nude mice s.c..When RCC-9863 passaged for 20 times,the tissue from the same xenotransplant tumor were used to construct SOI model.Cultured the metastatic tissue in vitro,the tumor cell suspension was then injected orthotopically.The metastatic tissue obtained underwent the same procedure again.At last,the metastatic tumor was cultured in vitro and cloned. Results 15 days later, a tumor mass sized 1.7 cm?0.6 cm in the nude mouse's renal parenchyma was grown which lobulated,rude,and with multiply blood vessels and 55 days later the mouse became moribund and metastases in the lungs were formed.The transplanted renal tumor in the SOI model grew fast and invasively and metastasized to lungs,lymphatic node and liver.A subline,MRCC,with metastatic ability to the lung was selected.Compared with RCC-9863,MRCC has the characteristics of shorter multiply time and higher agar clone forming rate(P
