Low resolutation fluorotyping of HLA-DRB1 locus
- VernacularTitle:荧光定量分型法在HLA-DRB1位点分型中的应用
- Author:
Xianyi LI
;
Jingzhong LIU
;
Juzhong GAO
- Publication Type:Journal Article
- Keywords:
Histocompatibility antigens;
Genes;
Spectrometry,fluorescence
- From:
Chinese Journal of Urology
1994;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate and evaluate a novel fluorotyping procedure for HLA-DRB1 locus. Methods On the basis of low resolutation SSP technique,the fluorogenic probe was used to establish the fluorotyping procedure of HLA-DRB1 which was carried out for 46 samples. Results The fluorotyping has been successful for all the 46 samples and was completely coincident to the SSP results.As compared to the serological typing of DR locus,the coincidence rate was 66.3%(61/92) whereas the coincidence rate of both the 2 specific genes was 43.5%(20/46). Conclusions Fluorotyping is accurate,sensitive,reproducible,depends less on manual manipulation and eliminates the problems related to contamination.
