Effect of human serum albumin on the attachment of human gingival epithelial cells to commercial pure titanium
- VernacularTitle:人血清白蛋白对人牙龈上皮细胞在纯钛表面粘附作用的影响
- Author:
Yu LIU
;
Shenggen SHI
;
Yingliang SONG
- Publication Type:Journal Article
- Keywords:
Human serum albumin;
Human gingival epithelial cells;
Cell attachment;
Titanium
- From:
Journal of Practical Stomatology
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
objective: To investigate the effect of human serum albumin(HSA)on the attachment of human gingival epithelial cells(HGEs)to commercial pure titanium(cpTi).Methods: HGEs were cultured, the cells of passage 2～5 were seeded on to cpTi samples which were preincubated in keratinocyte serum free medium with 50 mg/ml HSA or without HSA. Cell attachment was studied by immunofluorescence analysis.Results: HGEs were cultured and identified by positive expression of cytokeratin. 4,12 and 24 h after seeding attached cells on HSA preincubated cpTi were 218,730 and 849, those on the control 417,745 and 864, respectively.Conclusion: The early stage of cell attachment of HGEs on to cpTi may be inhibited by HSA.
