Inhibitary effects of hydroxycampothecin, pingyangmycin, cis-platinum or their combination on the human tongue carcinoma cells
- VernacularTitle:羟基喜树碱、平阳霉素、顺铂单独及联合应用对人舌癌细胞活性的影响
- Author:
Peng CHEN
;
Delin LEI
;
Moyi SUN
- Publication Type:Journal Article
- Keywords:
Hydroxycampothecin;
Pingyangmycin;
Cis platinum;
Tca8113 cell;
MTT assay
- From:
Journal of Practical Stomatology
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
objective: To study the effects of hydroxycampothecin(HCPT), pingyangmycin(PYM), cis platinum (DDP) or their combination on the proliferation of human tongue carcinoma Tca8113 cells. Methods: MTT assay, cell counting,clonogenic assay and HE staining were used to study the effects of HCPT, PYM, DDP or their combination on the proliferation of human tongue carcinoma Tca8113 cells. Results: ①Synergistic inhibiton was observed when HCPT at the concentration (ng/ml) of 10～100 combined with PYM at 32～320 or DDP at 32～320, antagonistic effect was observed when PYM at 32～320 combined with DDP at 32～320; ②After treatment with PYM, HCPT or PYM plus HCPT at IC 30 the clonogenecity (%) of the cells was 15.2, 11.6 and 4.1 respectively, while that of untreated cells 31.7. Conclusion: The combination of HCPT with PYM may be synergistic in the inhibition of tongue carcinoma cell proliferation.
