Preliminary Screen and Identification of One Nitrobacteria
- VernacularTitle:一株硝化杆菌的筛选及初步鉴定
- Author:
Jinlai ZHENG
;
Junwen LI
;
Fuhuan CHAO
- Publication Type:Journal Article
- Keywords:
Nitrobacter;
Polymerase chain reaction;
Oligonucleotide array sequence analysis
- From:
Journal of Environment and Health
1993;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen and seperate nitrobacteria that could be used to treat nitrogenous compound pollution.Methods Autotrophic nitrite-oxidizing microorganisms were screened by enrichment culture from soil.After morphological and biological detection,primers were designed to detect the distinctive gene norB of nitrobacteria.The PCR production was se-quenced,and the homology was identified according to the sequence in Genbank.Results One strain of bacterium was screened that could oxidize nitrite.This bacterium was small,light-yellow bacilli.The expected-size DNA fragments could be amplicated by PCR.The sequence of a PCR production was blasted in Genbank and showed99%consistent with norB gene of N hamburgensis.Conclusion It was preliminarily confirmed that the microorganism screened in this study might be nitrobac-terium.
