Experimented gene therapy for prostate cancer with HSV-TK/GCV system
- VernacularTitle:HSV-TK/GCV体系对前列腺癌PC-3m细胞杀伤作用的实验研究
- Author:
Dongqing ZHANG
;
Chunxiao XU
;
Daoxin MA
- Publication Type:Journal Article
- Keywords:
suicidal gene;
prostate cancer;
gene therapy
- From:China Oncology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Purpose: To investigate the effect of HSV-TK/GCV system on human prostate cancer. Methods: MTTassay, flow cytometry( FCM), optical and electron microscopy were used to determine the sensitivity of GCV on prostate cancer cell( PC-3m) after being tranfected with HSV-TK gene. Results: It was found that GCV had significant cytotoxic activity on HSV-TK gene-transduced prostate cancer cells, but little effect on the non-transduced cells. Conclusions: The experiment indicates that HSV-TK/GCV system has significant antitumor activities on the TK gene-transduced prostate cancer cells in vitro.
