Effects of gut peptides on the activation of mast cells from rat intestinal mucosa in vitro
- VernacularTitle:胃肠多肽对肠粘膜肥大细胞稳定性的影响
- Author:
Cheng LAN
;
Chengwei TANG
- Publication Type:Journal Article
- Keywords:
Substance P Somatostatin Vasoactive in testinal peptide Intestinal mucosal mast cells Histamine
- From:
Chinese Journal of Immunology
1999;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of substance P(SP),somatostain(SST) and vasoactive intestinal peptide(VIP) on the activation of rat intestinal mucosal mast cells(IMMC) in vitro.Methods:IMMC isolated and purified from the whole intestines of normal rats were incubated with gut peptides at various concentrations.The histamine concentration in IMMC and their supernate were determined.Furthermore,the ultrastructure of the incubated IMMC was observed under a transmission electronic microscope.Results:①The spontaneous release rate of histamine was (22.86?3.22)%.②SP significantly increased the histamine release rate from IMMC(P0.05)④At the concentration from 1?10 -1 mol/L to 1?10 -8 mol/L,the higher concentration of VIP was used,the lower histamine release rate was observed(P
