Primary culture and identification of human corpus cavernosum smooth muscle cells
- VernacularTitle:人阴茎海绵体平滑肌细胞的原代培养及鉴定
- Author:
Wenjun BAI
;
Shukun HOU
;
Xiaofeng WANG
- Publication Type:Journal Article
- Keywords:
Muscle,smooth;
Cells,cultured
- From:
Chinese Journal of Urology
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To provide an ideal material for the investigation of penile erection. Methods Human corpus cavernosum smooth muscle cells were cultured with DMEM ( 20% of it was human serum)in vitro and the bred cells were identified by immunohistochemistry. Results After 10～14 days, spindle cells overspreaded in 6 culture bottles and they were validated as human corpus cavernosum smooth muscle cells immunohistochemically. Conclusions Human corpus cavernosum smooth muscle cells can be cultivated in feasible medium and can also become a cell line.These bred cells can serve as a material for the study of penile erection.
