Rapid Detection of Non-01 Vibrio Cholerae in Haihe River by Vibrio Cholerae Outer Membrane Protein Gene (ompw) PCR Technology
- VernacularTitle:PCR快速测定法在检测海河水体非01群霍乱弧菌中的应用
- Author:
Jian_qing DING
;
Shu_mei WANG
;
Yong_qin TIAN
- Publication Type:Journal Article
- Keywords:
Gene;
Vibrio, cholerae;
Outer membrane protein primers;
Non_01Vibrio cholerae;
Gene amplification;
Haihe river water system
- From:
Journal of Environment and Health
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect pollution status of non_01 Vibrio cholerae in Haihe river by Vibrio cholerae outer membrane protein gene (ompw) PCR technology. Methods A pair of Vibrio cholera ompw primers was synthetized to detect non_01 Vibrio cholerae in 102 water samples collected from 5 sampling sites in Haihe river by ompw PCR approach and the results were compared with that of the routine bacterial separation culture method. When amplified by PCR, isolated non_01 Vibrio cholerae strain Vbo35 was used as positive reference, Shigella, E.coli and Salmonella strains were used as negative reference in the study. Results The total positive rate of Vibrio cholerae in 102 water samples was 52% and the amplified products were 588 bp. The detection results of the PCR approach were 100% in accord with that of the routine bacterial separation culture method. Conclusion The results indicated that ompw PCR technology was a highly sensitive and specific method to determination of non_01 Vibrio cholerae in Haihe river water system, it shortened the determination time 4～5 times, and could provided a useful determination method for non_01 Vibrio cholerae.
