Study on changes of proliferation and apoptosis of spermatogenic cell in idiopathic oligospermia
- VernacularTitle:原因不明少精子症睾丸生精细胞增殖与凋亡变化的研究
- Author:
Yaxuan WANG
;
Guangzeng CAI
;
Shuwen YANG
- Publication Type:Journal Article
- Keywords:
Oligospermia;
Proliferation cell nuclear antigen;
Apoptosis;
Genes
- From:
Chinese Journal of Urology
2001;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the pathophysiological mechanism of proliferation and apoptosis in spermatogenic cell of idiopathic oligospermia. Methods Forty one patients with oligospermia were included in the trial.Testicular tissues,which were obtained with Tru Cut needle biopsy,were observed under light microscope.Expressions of p16 and PCNA,incidence of apoptosis in spermatogenic cells were detected by immunohistochemistry and in situ 3′ DNA end labeling technique. Five testicular samples which were proved to be normal by examination served as controls. Results Proliferation index (21.6?13.1)% in testes of patients was lower than the normal controls (45.4?5.7)%, P
