The inhibitive effect of antisenes oligodeoxynucleotide on the phosphodiesterase type 5 in smooth muscle cells of human corpus cavernosum
- VernacularTitle:反义寡脱氧核苷酸对人阴茎海绵体平滑肌细胞PDE5的抑制作用
- Author:
Wenjun BAI
;
Shukun HOU
;
Xiaofeng WANG
- Publication Type:Journal Article
- Keywords:
Antisense oligodeoxynucleotide;
Phosphodiesterase type 5 Penis
- From:
Chinese Journal of Urology
2001;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibitive effect of antisenes oligodeoxynucleotide(ASON) on the phosphodiesterase type 5(PDE5) in smooth muscle cells of human corpus cavernosum,and provide experimental groundwork for the gene therapy of erectile dysfunction. Methods PDE5 gene ASON (containing exon 1) was transfected into the corpus cavernosum smooth muscle cells with the presence of liposome DOTAP.Another sense oligodeoxynucleotide(SON) and 1% of bovine serum were also transducted into the cells as controls.PDE5 was probed and measured by Western blot at 1,2,4,6,10,24 and 48 h after transfection. Results After transfection(1～6 h), PDE5 in human corpus cavernosum smooth muscle cells was significantly lower than it was in controls( P = 0.000 ～0.014). Conclusions The results indicate that PDE5 gene antisense oligodeoxynucleotide has inhibitive effect on PDE5 in smooth muscle cells of human corpus cavernosum in vitro,and the present study provides experimental groundwork for the gene therapy of erectile dysfunction.
