Expression of phosphodiesterase 5 in clitoral corpus cavernosum and effect of icariin on cGMP levels in vitro
- VernacularTitle:阴蒂海绵体中磷酸二酯酶5表达及淫羊藿甙对cGMP浓度的影响
- Author:
Liang QIAO
;
Zhongcheng XIN
;
Jie FU
- Publication Type:Journal Article
- Keywords:
Clitoris;
Phosphodiesterase 5;
cGMP;
Icariin
- From:
Chinese Journal of Urology
2001;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of phosphodiesterase 5 (PDE5) in clitoral corpus cavernosum and effect of icariin on cyclic GMP (cGMP) levels in vitro. Methods The presence of mRNA coding for PDE5 in clitoral corpus cavernosum was detected by means of RT PCR analysis.The effect of icariin with various concentrations on cGMP levels,which is the target of PDE5 in vivo,was measured by 125 I radioimmunoassay with sildenafil as positive control. EC 50 of icarrin and sildenafil was calculated. Results There was a lane around 800 bp on the agar electrophoresis of the production of RT PCR,which was as long as we designed according to cDNA sequence of PDE5.In the presence of SNP,icariin increased cGMP concentrations in rabbit clitoral corpus cavernosum with increasing dose. Conclusions The results support the proposal that icariin may influence cGMP level by NO cGMP signaling pathway as an inhibitor of PDE5.
