Hytk gene expression promoted by PSA promoters in prostate carcinoma cell lines
- VernacularTitle:带PSA启动子的hytk基因在前列腺癌细胞中的表达
- Author:
Chuanzhong YE
;
Ying YU
;
Bing SU
- Publication Type:Journal Article
- Keywords:
Prostatic neoplasms;
Carcinoma;
Genes
- From:
Chinese Journal of Urology
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study human prostate cancer tissue specific hytk gene therapy vector using prostate specific antigen (PSA) promoter,and to determine its feasibility. Methods A prostate tissue specific hygromycin phosphotransferase thymidine kinase fusion gene(hytk)vector was established with the substitution of CMV promotor in tgCMV/hytk vector with PSA promotor.The vector was transferred into prostate carcinoma cell lines with FuGENE TM 6.Antitumor effects were observed after GCV treatment. Results PSA promoter that actively drives hytk gene was expressed only in the PSA producing prostate cell line(LNCaP).In vitro experiments demonstrated the LNCaP cells transferred by HyTK gene were killed in the presence of GCV treatment. Conclusions Hytk based vector harboring PSA promoter is a novel ideal candidate vector for prostate carcinoma gene therapy.
