Effects of intermittent conventional and continuous pressure-controlled tissue expansion on cell proliferation and apoptosis
- VernacularTitle:持续恒压和间断常规组织扩张术对皮肤细胞增殖与凋亡的影响
- Author:
Jiang LI
;
Kaihua LU
;
Yufeng AI
- Publication Type:Journal Article
- Keywords:
Tissue expansion;
Skin;
Cell cycle;
Swine, miniature
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the cell dynamic changes of conventional intermittent tissue expansion (CITE) and continuous pressure controlled tissue expansion (CPTE). Methods Domestic pigs were chosen for CITE and CPTE models. Immunohistochemical technique was used to detect proliferative cells, DNA fragments in situ labeling for apoptotic cells, and H E stain for total fibroblast counting. Results Proliferative index of basal cell increased during expansion. The peak value of 78.5% reached at the ninth day in the CITE group versus 84.2 % at fourth day in the CPTE group. Proliferation index in both groups decreased after expansion. Fibroblast proliferation, apoptosis and total fibroblast density increased slowly in both groups during and after expansion. At the sixth day, total fibroblast densities in CITE group (38.1 cells/H) and in CPTE group (40.9 cells/H) were significantly increased, compared with 34.93 cells/H in the normal skin. There were obvious proliferation and apoptosis phenomena in epidermal cells and skin adnexa. Conclusion Tissue expansion has both effects of proliferation and apoptosis on cell dynamics. Continuous tissue expansion can induce tissues growing much effectively.
