Alternation in ?-adrenergic responsive cAMP of human detrusor and its regulation in the aged
- VernacularTitle:老年人膀胱逼尿肌?受体反应性环腺苷酸水平变化及其调节机制
- Author:
Zhenhua LI
;
Gang LI
;
Xu ZHAO
;
Ping WANG
- Publication Type:Journal Article
- Keywords:
Bladder;
Receptors, adrenergic beta;
Cyclic AMP
- From:
Chinese Journal of Geriatrics
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the influence of age on ?-adrenergic responsive cyclic adenosine monophosphate (cAMP) level of human bladder detrusor and its modulation mechanism. Methods The cAMP levels in detrusor muscle of aging group (15 cases) and young group (7 cases) were determined by radioimmunoassay at the presence of isoprenaline (ISO), BRL 37344 and forskolin, in addition, the modulation of cAMP content of aging group by pertussis toxin(PTX), cholera toxin(CTX), vinpocetine(VPT) and KT-5720 were also studied. Results After incubation with ISO, BRL 37344 and forskolin, the cAMP level of aging group was (15.0?1.6),(12.2?1.8)pmol/mg and (4.5?4.0) pmol/mg protein. The cAMP level of aging group was not influenced significantly by CTX; the ?-adrenergic responsive cAMP level was enhanced significantly by PTX and KT-5720, but the increment of cAMP content induced by forskolin was not changed; VPT significantly increased the cAMP levels induced by all the three agents. Conclusions There is age-related decline in the ?-adrenergic responsive cAMP level of detrusor muscle of the aging; reduced activity of adenylyl cyclase and enhanced activity of inhibitory G protein Gi may be the biochemical basis; inhibitors of calcium/calmodulin stimulated phosphodiesterase and protein kinase A improves the ?-adrenergic responsiveness of the senile, therefore might be used to treat the urinary storage dysfunction during aging.
