Expression of hepatocyte growth factor and c-met stimulated by high glucose in human kidney fibroblast and its significance
- VernacularTitle:高糖刺激人肾脏成纤维细胞肝细胞生长因子和c-met的表达及其意义
- Author:
Shan MOU
;
Qingyi ZHANG
;
Hanfang ZHAO
;
Jufang TONG
- Publication Type:Journal Article
- Keywords:
Kidney;
Fibroblasts;
Hepatocyte growth factor;
Proto oncogene protein c met;
Gene expression;
Diabetic Nephropathy
- From:
Chinese Journal of Endocrinology and Metabolism
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe high glucose induced expression of hepatocyte growth factor (HGF) and c met in human kidney fibroblast. Methods The effects of glucose concentrations on expression of HGF, c met and plasminogen activator inhibitor (PAI) 1 in cultured human kidney fibroblasts were observed by RT PCR. In the same system, the effect of exogenous HGF on the expression of PAI 1 was investigated. Results Human kidney fibroblasts cultured in high glucose concentration (25 mmol/L) showed higher HGF and c met expressions in the early stage and then manifested a gradient decrease of HGF and c met expressions, but PAI 1 expression was gradiently increased. Exogenous HGF resulted in inhibiting PAI 1 expression. Conclusion HGF is a potential anti fibrogenic factor and activates matrix degradation pathways in diabetic kidney by reducing PAI 1 expression.
