Study of planting isogenous Schwann cells into chemical extracted allogenous nerve in vitro
- VernacularTitle:化学萃取同种异体神经种植许旺细胞的体外实验
- Author:
Jianyun WANG
;
Xiaolin LIU
;
Jiakai ZHU
- Publication Type:Journal Article
- Keywords:
Schwann cells;
Peripheral nerve;
Chemical extraction
- From:
Chinese Journal of Microsurgery
2000;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To look for an ideal substance to repair large gap of nerve defect after injuries by culture,population of Schwann cells(Scs)and preparation of acellular allogenous nerve grafts with chemical extraction. Method The double adhesion culture and Arab c to prohibit the fibroblast growth were used to achieve high purified Scs;detergents:Triton X 100 and sodium deoxycholate were used to achieve acellural nerve grafts (ANG);and finally the Scs were micro injected into the acellural nerve grafts and the consequence studied Result By the way above,high purified Scs and the ANG was acquired,which can integrated each other well Scs can survived and transformed to aline in vitro Conclusion ANG populated isogenous Scs may be an ideal substance to repair the large gap of nerve defect after injuries
