The regulatory effects of PTK on neutrophils in response to transmembrane TNF-? and secreted TNF-?
- VernacularTitle:PTK对跨膜型与分泌型TNF-?诱导中性粒细胞功能的调节作用
- Author:
Li LI
;
Qingfen LI
;
Zhuoya LI
- Publication Type:Journal Article
- Keywords:
Transmembrane TNF-? Secreted TNF-? PTK Respiratory burst NO
- From:
Chinese Journal of Immunology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of PTK on the respiratory burst and release of NO by neutrophils in response to transmembrane TNF-?( TM-TNF-?) and secreted TNF-? ( S-TNF-?). Methods: The effects of PTK inhibitor on the functions of neutrophils caused by both forms of TNF-? was detected with the chemiluminescence and the nitrate reductase assay.The tyrosine phosphorylation of neutrophils induced by the two forms of TNF-? was compared by Western blotting analysis. Results: The PTK inhibitor genistein (50 ?mol/L) was found not only to depress the respiratory burst of neutrophils induced by S-TNF-?( P
