Study of the MxA protein induced by adenovirus type 3 and it' s antiviral effect against Ad3
- VernacularTitle:腺病毒3型诱导MxA蛋白产生及其抗病毒作用的研究
- Author:
Hairong GONG
;
Lun XU
;
Jicheng YANG
- Publication Type:Journal Article
- Keywords:
Adenovirus type 3 MxA protein Antiviral effect Hela cells
- From:
Chinese Journal of Immunology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: This study aimed to investigate the induction of MxA protein in PBMC treated with various doses of adenovirus type 3(Ad3) and to test the antiviral effect of MxA protein against Ad3 in vitro.Methods:The content of MxA protein in cytoplasm of the peripheral blood mononuclear cells( PBMC) , which were treated with various dose of Ad3 was detected by flow cytometry. The antiviral effect of MxA protein against Ad3 in Hela cells was studied by the microdose cytopathogenic effect inhibition assay. Results: MxA protein that in all the cell groups that were treated with various dose of Ad3 was higher than that of control. 10 ng/ml MxA protein can resist 20TCID50 Aad3. Conclusion: It was suggest that MxA protein that can be induced by Ad3 in PBMC and recombinant MxA can resist Ad3.
