The study of anti-tumor genecity effect of human IL-10 antisense and viral IL-10 antisense on nasopharyngeal carcinoma cells
- VernacularTitle:反义人IL-10和反义病毒性IL-10抗鼻咽癌细胞成瘤效应的研究
- Author:
Jianping TAO
;
Kun YANG
;
Zhenyu ZHU
- Publication Type:Journal Article
- Keywords:
Human Interleukin-10 Viral Interleukin-10 Antisense RNA Nasopllaryngeal carcinoma Tumorgenecity
- From:
Chinese Journal of Immunology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effectiveness of anti-tumorgenecity of human IL-10 antisense and viral IL-10 antisense on nasopharyn-geal carcinoma(NPC) cells.Methods:Human IL-10 cDNA and viral IL-10 gene had been amplifed and cloned. A two-valent eukaryotic expression vector for antisense nucleic acids of human IL-10 cDNA and viral IL-10 gene had been constructed and designated as pcDNA3/AS hIL-10 + AS vIL-10.The recombinant vector was transfected into a NPC-clerived cell strain-SUNE cells. After that, the transfected and nontransfected SUNE cells were inoculated i.d. in the right flank of SCID mice and hu-PBL-SCID mice to evaluate their tumorgenecity changes.Results:The results of ELISA analysis showed that the concentration of hIL-10 and vIL-10 was 242 ?3 pg'ml-1 in supernatant of the nontransfected SUNE cells, and onry 22 ? pg ?ml-1 of hIL-10 and vIL-10 contained in supernatant of the transfected SUNE cells. After inoculation to SCID mice, the transfected and nontransfected SUNE cell showed the same growth manner. But inoculating to hu-PBL-SCID mice, the transfected SUNE cells grew slowerly than the nontransfected SUNE cell. With statistical analysis, the size and mean weight of the tumors from the hu-PBL-SCID mice inoculated with transfected SUNE cells were significantly lower in contrast to that from the hu-PBL-SCID mice inoculated with nontransfected SUNE cells( P
