Application of HLA-DRB、DPB1 genotyping by PCR-SSP、RFLP for allogeneic bone marrow transplantation
- VernacularTitle:异基因骨髓移植中PCR-SSP和RFLP对HLA-DRB、DPB1分型的应用
- Author:
Xianghua UN
;
Zhizhe CHEN
;
Minhui LIN
- Publication Type:Journal Article
- Keywords:
PCR-SSP RFLP HLA antigens Genotyping Allo-BMT
- From:
Chinese Journal of Immunology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the feasibility of clinical application of HLA-DRB,DPB1 genotyping by PCR-SSP,RFLP for alloge-neic bone marrow transplantation(Allo-BMT) .Methods: Corresponding to each of the DRB alleles, nineteen different pairs of sequence specific primers were used to PCR amplify them from twenty samples in both recipients and donors and their amplified products were directly analyzed on agarose gel. At the same time, a pair of primer was used to PCR amplify the second exon of DPB1 and the restriction fragment length polymorphisms were analyzed on PAGE after their PCR products were separately digested by ten endonucleases. Results: Each DRB was typed in according to its clear and visualized electrophoresis band; the DPB1 genotype was also determined by analysis of all codes representing polymorphism fragment band with a computer. Four couples of recipient and donor were matched. Conclusion:PCR-SSP and RFLP are rapid, accurate and reliable genotyping approaches for Allo-BMT.
