Expression of the Bcl-2 and Bax mRNA in eosihophils of BALF in guinea pig with asthma
- VernacularTitle:哮喘豚鼠嗜酸细胞Bcl-2和Bax mRNA表达及意义
- Author:
Zhikui LI
;
Changzheng WANG
;
Guisheng QIAN
- Publication Type:Journal Article
- Keywords:
Asthma Eosinophils Bcl-2 Bax Apoptosis
- From:
Chinese Journal of Immunology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the relation between expression of Bcl-2,Bax and eosinophils(Eos) apoptosis,explore effect of Bcl-2 and Bax on asthma.Methods:Guinea pigs were divided into normal and asthmatic group randomly. Ovalbumin was used to prepare model of asthma in guinea pig. Apoptosis of Eos in BALF was detected.mRNA expression of Bcl-2 and Bax in Eos were measured by hybridization and RT-PCR.Results:Comparing with normal group,Eos in BALF from asthmatic group were significantly higher; the percentage of apoptotic Eos from asthmatic group were significantly lower( P
