Quantitative analysis of p75 ~( NTR) and hyperphosphorylated tau containing neurons in the hippocampal CA1 subfield of Alzheimer disease
- VernacularTitle:阿尔茨海默病海马CA1区p75~(NTR)和磷酸化tau蛋白神经元的定量观察
- Author:
Xiangyou HU
;
Hao XU
;
Jiangning ZHOU
- Publication Type:Journal Article
- Keywords:
Alzheimer disease;
Immunohistochemistry;
Hippocampus;
Receptor, nerve grouth factor;
tau Proteins
- From:
Chinese Journal of Neurology
2000;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate p75 NTR expressing neurons and hyperphosphorylated tau protein containing neurons in the hippocampal CA1 subfield of Alzheimer's disease. Methods Samples of hippocampus of 10 female AD patients and 10 non-demented female controls matched with age and postmortem delay were obtained from the Netherlands Brain Bank. The main body of hippocampus was dissected, dehydrated and embedded in paraffin. Serial 6-?m coronal sections were cut, and 3 successive sections were selected. The first section was stained with 0.5% thionin, the second was processed for p75 NTR immunocytochemistry and the third was processed for p75 NTR double-labeling immunocytochemistry with Alz-50. For quantitative analysis of the total number of neurons, p75 NTR expressing neurons and neurons colocalizing p75 NTR and Alz-50 in the CA1 subfields, a MetaMorph image acquisition and processing software (Universal Imaging Corp, USA) was used.Results The total number of neurons, p75 NTR immunoreactive neurons and ratio of the latter to the former in 1 mm2 of the CA1 subfield of AD patients were 293.2?37.0, 116.0?20.4 and 39.7%?5.3%, respectively, of controls were 473.6?63.1, 136.7?24.4 and 28.9%?3.7%, respectively. There was significant decrease in the total number of neurons of the AD patients in comparison with controls (P=0.000 2). However, the ratio of p75 NTR neurons to total number of neurons was significant increase in AD patients compared with controls (P=0.001). The number of Alz-50 neurons, Alz-50 and p75 NTR double-labeling neurons and ratio of the latter to the former in 1 mm2 of the CA1 subfield of AD patients were 87.5?29.2, 76.4?26.6 and 86.6%?5.0%, respectively. Furthermore, the number of p75 NTR containing neurons was significantly correlated to the number of Alz-50 expressing neurons (r=0.79, P=0.006). Conclusion The p75 NTR may interact with hyperphosphorylated tau protein and involve in the pathogenesis of Alzheimer′s disease.
