Injection of bradykinin to hippocampus induces Alzheimer-like phosphorylation of tau and abnormal behavior in rat
- VernacularTitle:海马注射缓激肽复制阿尔茨海默病神经原纤维变性动物模型
- Author:
Quanbao WANG
;
Jianzhi WANG
- Publication Type:Journal Article
- Keywords:
Alzheimer disease;
Ca(2+)-calmodulin dependent;
Protein kinase;
Bradykinin;
Neurofibrillary tangles;
tau Proteins
- From:
Chinese Journal of Neurology
2000;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To reconstitute an animal model based on the imbalance of protein kinase(s) and protein phosphatase(s) seen in Alzheimer brain. Methods The injection of bradykinin (BK) into hipocampus was performed, and their behaviors were observed by electronic attack-jump experiment and phosphorylation of tau using immunohistochemical assay. Results The results of behavior studying showed that an obvious disturbance in learning and memory was seen in BK injected rats. The results obtained by immunohistochemical assay indicated that the staining for M4?12E8?PHF-1 and CaMK-Ⅱ was stronger, and for tau-1 was weaker in BK injected rats as compared with the control group. The BK injected rats showed obvious deficit in behavior [mistakes made by model and control rats during electronic attack-jump experiment:8.3?2.5 and 6.9?3.1, P
