Caspase-3 in cellular apoptosis of bladder carcinoma induced by mitomycin C
- VernacularTitle:半胱氨酸蛋白酶32在丝裂霉素C诱导膀胱癌细胞凋亡中的作用
- Author:
Changlin ZHAO
;
Huimian XU
;
Jia LIU
- Publication Type:Journal Article
- Keywords:
Bladder neoplasm;
Carcinoma;
Cysteine synthase;
Cell apoptosis
- From:
Chinese Journal of Urology
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate caspase 3 in the apoptosis of EJ cells in bladder carcinoma induced by mitomycin C(MMC). Methods The apoptosis and changes in cell cycle were examined by means of TUNEL and flow cytometry. The ability of caspase 3 antibody to resist apoptosis induced by a low dose of mitomycin was also studied. Results The typical characteristics of apoptosis were observed in EJ cells treated with low dose of mitomycin and the apoptotic index (AI) was (62.9? 2.2 )%, being much higher than that in the group treated combinedly with caspase 3 antibody and MMC(4.9?0.3)% and in the controls (2.7?0.7)%, P
