Construction of alopecia areata dermal papilla cDNA substractive library
- VernacularTitle:应用抑制性消减杂交方法构建斑秃毛乳头差异表达基因文库
- Author:
Xichuan YANG
;
Fei HAO
;
Bo CHENG
- Publication Type:Journal Article
- Keywords:
Alopecia areata;
Genes;
Hybridization
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct a cDNA substractive library of dermal papilla cell (DPC) in patchy and normal area of alopecia areata with technique called suppression substractive hybridization. Methods Total mRNA was isolated form DPC of patchy and normal area. Moreover, double strand cDNAs were synthesized in turn. cDNA from patch and normal area then were restricted and divided into two groups and ligated to the specific adaptor 1 and adaptor 2 respectively. After cDNAs from patchy and normal area hybridized with each other twice and underwent two times of nested PCR, then PCR products were ligated with arms of T/A plasmid vectors to set up the substractive library. Results cDNA substractive library of DPC in patchy and normal area of alopecia areata was set up successfully with highly subtractive efficiency. The amplified library contained 120 positive clones, in which 90 positive subclones contained 100 500bp inserts. Conclusion The cDNA substractive library of DPC in patchy and normal area of alopecia areata is a highly efficient one and lays a solid foundation for screening and cloning new and specific genes from patchy and normal DPC of anagen hair follicle in DPC of alopecia areata.
