ELISA using Torpedo Acetylcholinesterase labelled second antibody
- VernacularTitle:电鳐乙酰胆碱酯酶标记第二抗体酶联免疫吸附测定法
- Author:
Qian LI
;
Wei LIU
;
Fengzhen LI
- Publication Type:Journal Article
- Keywords:
Torpedo AChE NaIO4-mediated glycoprotein oxidation method AChE-antibody conjugates
- From:
Chinese Journal of Immunology
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:In order to build the method to apply Torpedo Acetylcholinesterase(AChE) labelled second antibody in ELBA. Methods:The labelling method is NaIO4-mediated glycoprotein oxidation method. Results:pH and temperature and the concentration of NaIO4 effect on labelling efficiency. The liter of AChE labelled IgG is higher than HPR labelled IgG. Conclusion:Torpedo Acetylcholinesterase-labelled second antibody prepared could be applied and replaced the HRP-labelled second antibody in ELISA.
