Influence of histamine on interleukin-2 production and proliferation of T lymphocytes in vitro
- VernacularTitle:组胺对T细胞IL-2产生及增殖活性影响的实验研究
- Author:
Guohua YIN
;
Kaiming ZHANG
;
Jiangyang WEN
- Publication Type:Journal Article
- Keywords:
Histamine T lymphocyte Interleukin-2 Proliferation
- From:
Chinese Journal of Immunology
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the influence of histamine on intedeukin(IL-2) production and proliferation of CD4+ T lymphocytes and CD8+ T lymphocytes.Methods:Density gradient centrifugalization and absorption technique were respecitively applied to detach peripheral blood mononuclear cells(PBMC) and peripheral blood lymphocytes(PBLC). CD4+ and CD8+ T lymphocytes divided with anti-CD4+ and anti-CD8+ antibody were cultured in vitro. IL-2 levels in supernatant were quantified by enzyme-linked immunosobent assay (ELBA), and proliferation index were measured by MTT methods. Results:IL-2 levels in supernatant and proliferation rate of CD4+ and CD8+ T lymphocytes treated with histamine were significantly lower than those of their corresponding un-stimulated controls(P
