The study of effects of adenovirus-mediated p16 gene transfer on cell growth of the human bladder carcinoma in vitro
- VernacularTitle:腺病毒介导的p16基因转染对膀胱癌细胞生长影响的研究
- Author:
Wenquan ZHOU
;
Zhilian MIN
;
Jianping GAO
- Publication Type:Journal Article
- Keywords:
Bladder neoplasms;
Carcinoma;
Genes p16
- From:
Chinese Journal of Urology
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of full length p16 gene transfer by replication-defective recombinant adenoviruses on cell growth of the human bladder carcinoma. Methods Ad-p16 was infected into the human bladder carcinoma cell line with no expression of p16 protein,T24.The transfection efficiency of the recombinant adenoviruses was tested using Ad-LacZ by X-gal staining.The expression of p16 protein was evaluated by Western-blot analysis. The effects of Ad-p16 infection on cell proliferation were evaluated by MTT assay and flow cytometry. Results Higher transfection efficiency(97%) to T24 could be obtained at MOI(multiplicity of infection) of 50.This Ad-p16 has a high gene expression in T24 cells.The proliferation of Ad-p16 infected T24 cells was significantly suppressed as compared with that of Ad-LacZ-infected and non-infected cells ( P
