The functional changes of splenic lymphocytes in immobihzation stress mice
- VernacularTitle:束缚应激小鼠脾淋巴细胞功能的改变
- Author:
Yong MENG
;
Subin LIU
- Publication Type:Journal Article
- Keywords:
Immobilization stress Lymphocytes
- From:
Chinese Journal of Immunology
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the affection of immobilization stress (IMS) on mice splenic lymphocytic function. Methods:The proliferation of lymphocytes was examined by MTT, and intracellular[Ca2+] was examined by fluorescence probe, Fura-2/AM, membrane of lymphocytes IL-2 receptor(mIL-2R) and cell cycle were examined by flow cytometry. Results;The end of IMS was found to be the lowest proliferation response after IMS, at the same time mIL-2R expression was impaired. Analysis of cell cycle showed the proliferation of splenocytes from IMS mice might be bloched in G2/M phases. Intracellular[Ca2+] was significant increased after IMS. Conclusion: IMS may induce the disfunction of lymphocytes and immunosuppression.
