Establishment and characterization of a human renal carcinoma cell line RCC-9863
- VernacularTitle:人肾透明细胞癌细胞系RCC-9863的建立及其生物学特性
- Author:
Pengfei WANG
;
Changhong SHI
;
Chen SHAO
- Publication Type:Journal Article
- Keywords:
Kidney neoplasms;
Carcinoma;
Cells,cultured
- From:
Chinese Journal of Urology
2001;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a new renal cancer cell line so as to provide an useful tool for the basic research of renal cancer. Methods A renal tumor specimen sized 1.0 cm?1.0 cm was excised and inoculated subcutaneously into the right posterior leg of three nude mice.After three passages in the nude mouse,the tumor tissue was cultured in vitro. Following the reference, the identification of cell line were fulfilled. Results Two of the transplanted tumors grew in the nude mice and then part of the tumor was cut and inoculated again into nude mouse for another two generations. The tissue cultured in vitro was followed and finally a stable growth renal carcinoma cell line was established without change of morphological structure and differentiation. The mode rage of chromosome has been 62～68 (75%) and the cycle analysis showed G1 63.8%, G2 11.4% and S1 24.8%.The doubling time has been 37.7 hours.The tumor cell can secrete IL 6 continuously. Conclusions The renal carcinoma cell line RCC 9863 was identical to the primary cancer cell in biological characteristics.It has been cultured in vitro continually for more than one year with its characteristics remained.So,RCC 9863 is considered to be a stable cell line.
