The inhibiting mechanism of MMC on bladder cancer cell line EJ
- VernacularTitle:丝裂霉素对膀胱癌EJ细胞作用机制的研究
- Author:
Lili LIANG
;
Qiang ZHANG
;
Chung LEE
- Publication Type:Journal Article
- Keywords:
Bladder neoplasms;
Carcinoma;
MMC;
Apoptosis;
Necrosis
- From:
Chinese Journal of Urology
2001;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To present our attempt on investigate the effect of MMC on a human bladder cancer cell line, EJ cells. Methods EJ cells were maintained under tissue culture conditions in the presence of Mitomycin C (MMC), which had a potent cytotoxic effect on these cells. The apoptosis and necrosis of EJ cells were assessed and by using flow cytometry (FCM) ,TUNEL technology and eosin exclusion assay . Results Apoptosis of bladder cancer cell line EJ could be observed within the 24 h after the use of MMC in different doses.The apoptosis peak appeared in the 24th hour and a dose dependment effect could be observed. Conclusions MMC could inhibit the growth of bladder cancer cell via inducing apoptosis and necrosis of the cells.This might enclose the therapeutic mechanism of MMC on bladder cancer.
