Morphological changes of melanocytes in the epidermis of skin autografts
- VernacularTitle:全厚皮片移植后表皮黑素细胞形态学变化
- Author:
Haiyang LI
;
Wuxiu LI
;
Lifang WANG
- Publication Type:Journal Article
- Keywords:
Skin transplantation;
Melanocytes
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relationship between morphological and functional changes of melanocytes and their effect on the graft color. Methods Eighteen black whited guinea pigs were divided into nine groups randomly and biopsied at 1 d, 2 d, 3 d, 7 d, 14 d, 21 d, 28 d, and 90d after skin grafting, respectively. Then skin spliting and Dopa reaction techniques were used to demonstrate melanocytes. And computer assitant imagine analysis system was applied to observe and quantitate the morphological changes of melanocytes at various time points in three postoperative months. Results During three days after grafting, resulting from the surgery wound and shortage of blood and oxygen supply, the processes of the melanocytes broke off, accompanied by reduction of whole dimension of the cells, size as well as number of the processes. The indices above returned to the normal level between second week and third week after grafting. It was an evident characteristic that number of melanocytic processes became shorter and stouter in the end of the experiment. conclusion Functional changes of melanocytes in the skin grafts are in accord with their morphological changes. Compared to those in the sizes of melanocytes, changes in the processes of the cell are more sensitive in reflection of function of melanocytes. The shorter and stouter processes of melanocytes in the end of the experiment are suitable to the smaller epidermal melanin unit and more efficient in transformation of melanin into the keratinocytes.
