Preparation of human cementum extract and assay of its protein content
- VernacularTitle:人牙骨质基质提取物的提取及蛋白组分分析
- Author:
Yonglong HONG
;
Jianhua WEI
;
Baolin LIU
- Publication Type:Journal Article
- Keywords:
Dental cementum;
Extract;
Protein content
- From:
Journal of Practical Stomatology
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To prepare human cementum extract and to assess its protein content. Methods: Human permanent teeth were collected from the oral surgery clinic. Cementum was obtained from freshly extracted healthy teeth without inflammation.Connective tissue adhereing to the root surfaces was removed, and cementum was harvested with a curette. Then the cementum was treated by CH 3COOH and guanidine respectively to obtain two extracts. Protein content was assessed by sodium dodecyl sulphate ( SDS) polyacrylamide electrophoresis. Results: Extract obtained from CH 3COOH treatment consisted of two kind of proteins ( M r 55 000 and M r 68 000); that of guanidine consisted of four kind of proteins ( M r 62 000, M r 60 000 , M r 5 5000 and M r 41 000).Conclusion: The methods used is an applicable way to prepare cememtum extracts.
