Construction and erpression of NDV pIRHN nucleic acid vaccine and its effect onthe tumor cells
- VernacularTitle:新城疫病毒pIRHN核酸疫苗构建和表达及对肿瘤细胞的影响
- Author:
Lijuan XUE
;
Wei GONG
;
Tao GE
- Publication Type:Journal Article
- Keywords:
Sialic acid Newcatle disease virkus NDV HN gene Antitumor effect Hela Cell pIRHN nucleic Acid Vaccines
- From:
Chinese Journal of Immunology
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Abstract Objective:To explore antitumor effect of NDV HN. Methods: pIRHN nucleic acid vaccin was constructed and was transfectedto Hela cells. Westem blot was used to analyssize the expression of pIRHN nucleic acid vaccine in eukarytic cell. The mode of cell death was de-tected by fluorescence microscope, gel electrophoresis and TUNEL assay. The effect of pIRHN nucleic acid vaccine on the contents of siaha acidin the Hela cell was examined. Results: pIRHN nucleic acid vaccine could be expressed in the eukarytic cell. pIRHN could induced apoptosisafter HeLa cells were transfected. The effect of antitumor responses of pIRHN was correlated with the contents of sialic acid in tumor cells. Con-clusion: The reults of our experiment will provide the theory evidence for the anti tumor mechamism of NDV.
