The Oxidative Stress Way Induced by Soot on Rat Pulmonary Cells
- VernacularTitle:煤烟对大鼠肺细胞氧化性损伤作用途径的探讨
- Author:
Yunhui LI
;
Yuepu PU
;
Weijian ZHONG
- Publication Type:Journal Article
- Keywords:
Soot;
Free radical;
Pulmonary cell;
Anti-oxidative agent;
Oxidative Stress
- From:
Journal of Environment and Health
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
ve To study the way of oxidative stress induced by soot rat pulmonary cells type Ⅱ ?Methods Before exposure to soot extracts, the rat pulmonary cells were pretreated with anti-oxidative agent, N-acetylcysteine (NAC), vitamin C, vitamin E and mannitol for 30 minutes, then the ceils were exposed to the soot extracts for 24 hours. MTT assay was applied to detect the cytotoxicity. Results It showed that NAC, vitamin C and vitamin E could reduce the cytotoxicity of rat pulmonary cell caused by soot, but no same change was observed in that of man-nitol. Conclusion It was suggested that the DNA oxidative stress of soot may be caused by multi-ways, such as in-duction of oxygen free radical and lipid peroxide etc.
