Expression of ?_3 -adrenoceptor and its function in human detrusor cells
- VernacularTitle:人逼尿肌细胞中肾上腺素能?_3受体的表达及功能
- Author:
Ping WANG
;
Zhenhua LI
;
Gang LI
- Publication Type:Journal Article
- Keywords:
Urogenital system;
Receptors,adrenergic,beta;
Detrusor
- From:
Chinese Journal of Urology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of ? 3 adrenoceptor (? 3 AR) and its function in human detrusor cells. Methods Cellular functional studies,radioligand binding experiments and reverse transcription polymerase chain reaction were used to determine the expression and its influence on detrusor contractions. Results mRNA of 3 subtypes of ? ARs was detected. Non selective ? AR agonists and ? 3 AR decreased the contractions of detrusor cells induced by KCL, but both agonist and antagonists of ? 1 and ? 2 AR could not influence the contraction;BRL37344 concentration dependently displaced dihydroalprenolol binding to the membrane of detrusor cells. Conclusions The mRNA of subtypes of ? ARs is distributed in human detrusor cells,but ? 3 ARs is the main receptor mediating detrusor relaxation.
