Influence of Decoction Time on The Total Count of The Total Free Anthraquinone in Radix et Rhizoma Rhei
- VernacularTitle:煎煮时间对生大黄中五种游离蒽醌的影响
- Author:
Yongtao LIU
;
Hong ZHANG
;
Zhou HANG
- Publication Type:Journal Article
- Keywords:
Radix et Rhizoma Rhei;
Free Anthraquinone;
HPLC
- From:
Journal of Zhejiang Chinese Medical University
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To determine the relation between the total count of the five free anthraquinone(A loe-emodin,Rhein,Emodin,Chrysophanol,Physcion) in Radix et Rhizoma Rhei and the decoction time.[Method]HPLC was used with the chromatographic conditions as follows:mobile phase:methanol-0.1% phosphonic acid solution = 85 :15,detection wave length:254nm,flow rate:1.0 ml/min,column temperature:room temperature.The count of the five free anthraquinone in the decoction taken in every 2 minutes after the solution boiled was determined.[Results]During the 22 minutes after the solution was boiled,the total count took on a ascending tendency,afterwards,it took on a descending tendency.[Conclusion]The biggest number of the total count was obtained 20min after the solution was boiled.
