Effect of neuroprotectant combined with cocktail on focal cerebral ischemia in rats
- VernacularTitle:神经保护剂的鸡尾酒疗法对大鼠局灶性脑缺血后梗死体积和神经元凋亡及bcl-2蛋白表达的影响
- Author:
Dianqing PAN
;
Zhimei LI
- Publication Type:Journal Article
- Keywords:
Cerebral ischemia;
Neuroprotective agents;
Drug therapy, combination
- From:
Chinese Journal of Neurology
2001;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether cocktailtherapy combined with of neuroprotectants may have more advantages over single agents in treating focal cerebral ischemic cascade Methods Use suture occlusion technique, the right middle cerebral artery in rats was occluded Tirty minutes later,Fructose 1,6 diphosphate(FDP)(50 mg/kg, n =20), MK 801(1 mg/kg, n =20) and N acetylcystein(NAC)(150 mg/kg, n =20) were singly or combinantly infused intraperitoneally At the same time the cocktail treated group ( n =20) were infused the above agents combinationtly and the control group ( n =20) were infused normal saline intraperitoneally. six and 24 hours after focal cerebral ischemia,the animals were weighted and neurologically assessed on 5 point scale The animals were killed; brain were stained with 2,3,5 triphenylte trazolinm chloride for assessment of the volume of infarction, and then embedden onto slides with paraffin for morphological assessment and terminal transferase dUTP nick ending labeling(TUNEL) were carried out for apoptosis and immunohistochemistry were carried out to investigate the changes in bcl 2 Results All neuroprotectants decreased the volume of infarction( P
