Gene transfer of TNF receptor enhances the tumorcidal activity of TNF
- VernacularTitle:基因转移对肿瘤坏死因子杀伤肿瘤细胞作用的实验研究
- Author:
Xiaowen SUN
;
Li LIU
;
Dongliang YAN
- Publication Type:Journal Article
- Keywords:
Bladder neoplasms;
Tumor necrosis factor receptor;
Genes
- From:
Chinese Journal of Urology
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the influence of gene transfer of TNFR on the tumorcidal activity of TNF. Methods By constructing retroviral expressin vector of TNFR and transfecting package cell PA317,the virus that can transfer TNFR was established.Then the virus was used to infect BIU 87 cell line.The TNFR number on tumor cell membrane and the tumorcidal activity of TNF in vitro were detected. Results The number of TNFR on cell membrane of BIU 87 cell was 912/cell and increased to 2 872/cell( P
