Effect of RGD-insulin on activities of bone resorption and the possible mechanism in human osteoclast-like cells in vitro
- VernacularTitle:RGD胰岛素对人破骨样细胞骨吸收功能与机制的研究
- Author:
Dong LI
;
Yan XUE
- Publication Type:Journal Article
- Keywords:
Insulin;
Osteoma;
Osteoclasts;
Apoptosis
- From:
Chinese Journal of Geriatrics
1995;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective In this study, we explored the mechanism of anti-bone resorption of RGD-insulin using osteoclast-like cells (OLCs) from giant cell tumor of bone as an in vitro model. Methods The function of bone resorption was observed and the staining for tartrate-resistant acid phosphatase (TRAPase) was identified. The carbonic anhydrase Ⅱ (CAⅡ) gene expression, apoptosis and the adhesion of OLC that treated by RGD-insulin and echistatin were determined by in situ hybridization, TUNEL staining and adhesion test, respectively. Results OLCs were TRAP positive and had bone resorptive function. After OLCs were treated with 10 -5, 10 -6, 10 -7mol/L RGD-insulin and echistatin respectively, the numbers of TRAP-positive multinucleated cells were (179?3), (238?11), (351?10) per well that treated with echistatin and (229?20), (222?21), (265?17) per well that treat with RGD-insulin.The expression of CAⅡ mRNA decreased, the number of OLCs apoptosis increased in a dose-dependent manner (P
