Poly-beta-hydroxybutyrate as a scaffold for tissue-engineered cartilage
- VernacularTitle:应用聚羟基丁酸酯支架构建组织工程软骨
- Author:
Weidong YANG
;
Ping HU
;
Qichun QIAN
- Publication Type:Journal Article
- Keywords:
Tissue engineering;
Cartilage;
Polymer
- From:
Journal of Practical Stomatology
1996;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
?Objective: To investigate the feasibility and of poly beta hydroxybutyrate (PBH) as a scaffold for chondrocytes to fabricate tissue engineered cartilage. Methods: PBH was manufactured into porous scaffold with 85% porosity and the diameter of the pore was 300 400 ?m, and then the scaffold was modified with 100 mg/L human fibronectin.Chondrcytes(4?10 7/ml) harvested from auricular cartilage of rabbits were seeded into the PHB scaffolds. The PBH/chondrocytes composites were translated in the dorsal subcutaneous tissue of 6 New Zealand rabbits . PBH scaffolds without cells were used as the controls. The specimens were harvested and examined histologically . Results: Four and eight weeks after transplantation, the PBH/chondrocytes composites showed the morphology of cartilage. New cartilage tissue was formed with a little matrix in four weeks. In eight weeks the newly formed cartilage tended to be mature with more matrix . Conclusion: PBH can be used as scaffold for tissue engineered cartilage.
